PCR Protocols: A Guide to Methods and ApplicationsMichael A. Innis, David H. Gelfand, John J. Sninsky, Thomas J. White Academic Press, 02.12.2012 - 482 Seiten The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.
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Inhalt
RESEARCH APPLICATIONS | 167 |
GENETICS AND EVOLUTION | 259 |
DIAGNOSTICS AND FORENSICS | 323 |
INSTRUMENTATION AND SUPPLIES | 427 |
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PCR Protocols: A Guide to Methods and Applications Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White Keine Leseprobe verfügbar - 1990 |
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1990 by Academic Acad Academic Press agarose gel aliquots alleles amplification amplified DNA analysis annealing assay cDNA cells chromosome cloning concentration containing cycles denaturation detection digestion DNA fragments DNA sequences dNTP dot blot EDTA efficient enzymatic amplification ethanol ethidium bromide extraction Faloona first form reserved G. T. Horn gene genetic genomic genomic DNA Guide to Methods H. A. Erlich Higuchi hybridization Incubate labeled lanes Literature Cited membrane Methods and Applications MgCl2 minutes molecular molecules mRNA mutations Natl nonspecific Nucleic Acids Res nucleotide oligomer oligonucleotide probes PCR amplification PCR primers PCR product PCR Protocols Perkin-Elmer Cetus phage pmol polymerase chain reaction polymorphisms primer primer pair procedure quences reagents recombinant region restriction enzymes reverse transcriptase rights of reproduction rRNA Saiki Scharf single-stranded Sninsky solution Southern blot specific strand synthesis Taq DNA polymerase Taq polymerase temperature template tion tissue transcription tube virus vitro